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genome-wide methylation microarray analysis  (INFINIUM Inc)

 
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    INFINIUM Inc genome-wide methylation microarray analysis
    (A) To validate the changes in DNA <t>methylation,</t> the samples were re-analyzed with an Infinium <t>microarray</t> using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.
    Genome Wide Methylation Microarray Analysis, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome-wide+methylation+microarray+analysis/pmc03584082-133-5-9?v=INFINIUM+Inc
    Average 90 stars, based on 1 article reviews
    genome-wide methylation microarray analysis - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Curcumin Modulates DNA Methylation in Colorectal Cancer Cells"

    Article Title: Curcumin Modulates DNA Methylation in Colorectal Cancer Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0057709

    (A) To validate the changes in DNA methylation, the samples were re-analyzed with an Infinium microarray using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.
    Figure Legend Snippet: (A) To validate the changes in DNA methylation, the samples were re-analyzed with an Infinium microarray using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.

    Techniques Used: DNA Methylation Assay, Microarray, Modification, CpG Methylation Assay, Biomarker Discovery, Methylation



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    genome-wide methylation microarray analysis  (INFINIUM Inc)
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    INFINIUM Inc genome-wide methylation microarray analysis
    (A) To validate the changes in DNA <t>methylation,</t> the samples were re-analyzed with an Infinium <t>microarray</t> using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.
    Genome Wide Methylation Microarray Analysis, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome-wide+methylation+microarray+analysis/pmc03584082-133-5-9?v=INFINIUM+Inc
    Average 90 stars, based on 1 article reviews
    genome-wide methylation microarray analysis - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) To validate the changes in DNA methylation, the samples were re-analyzed with an Infinium microarray using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.

    Journal: PLoS ONE

    Article Title: Curcumin Modulates DNA Methylation in Colorectal Cancer Cells

    doi: 10.1371/journal.pone.0057709

    Figure Lengend Snippet: (A) To validate the changes in DNA methylation, the samples were re-analyzed with an Infinium microarray using independently bisulfite modified genomic DNA. The figure represents the number of CpG loci that showed CpG methylation changes with a Δβ-value of ≥0.1 in both experiments. 5-aza-CdR and TSA treated cells were used as positive and negative controls of validation. (B) Quantitative MSP (qMSP) was performed to validate the methylation changes in HCT116. Relative methylation was calculated by normalization of the methylation status of curcumin and 5-aza-CdR treated cells to controls. (C) The Venn diagram shows that CpG methylation changes overlap between HCT116, RKO and HT29 after the treatment with curcumin.

    Article Snippet: First, we performed an independent genome-wide methylation microarray analysis (Infinium®) using matched independent bisulfite modified DNA samples from curcumin and control cell lines.

    Techniques: DNA Methylation Assay, Microarray, Modification, CpG Methylation Assay, Biomarker Discovery, Methylation